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[DOI] [PMC free article] [PubMed] [Google Scholar] 41.Wei W, Sun W, Yu S, Yang Y, Ai L

The treated cells were lysed with radioimmunoprecipitation assay (RIPA) buffer [50 mM TrisHCl (pH 8.0), 0.05 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) SDS, and 150 mM NaCl] containing protease inhibitor cocktails (Merck, USA) with a ratio of 1:1000