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qPCR analysis was performed to measure the mRNA level of c-Fos ( g ), c-Jun ( h ) and Smurf1 ( i ) in indicated cells (n = 4 technical repeats from one of three independent experiments)

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Start with 50mcg administered once, preferably on a night when you can sleep in the next morning

Int J Mol Sci 23:4259

Plasticity of the murine spleen T-cell cholinergic receptors and their role in in vitro differentiation of nave CD4 T cells toward the Th1, Th2 and Th17 lineages