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2.6 Immunophenotyping by flow cytometry PBMCs were isolated using Ficoll-Paque density gradient centrifugation, frozen in freezing medium [10% DMSO, 90% fetal bovine serum (FBS)] and stored at -80C until use

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[9] described a case of motor paralysis after C4-5 posterior cervical foraminotomy, arguing that C5 nerve roots usually cover the entire intervertebral disc space and, therefore, require more excessive retraction than other nerve roots

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Botox-like peptides are bioengineered amino acid chains that reduce expression-induced wrinkles by interfering with neuromuscular communication in the skin