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3 ): Here, R red and R ox are the fluorescence ratios under fully reduced (DTT-treated) and fully oxidized (H2O2-treated) conditions, respectively, while I 488red and I 488ox are the fluorescence intensities at 488 nm in reduced and oxidized states

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in colon tissue, assessing NO levels and oxidative stress (MDA levels) (as result of the lysis of endothelial cells[9,10]) and ischemia/reperfusion injury, both as a spontaneous course [when blood supply was deprived via ligation (IC rats)] and a more exaggerated course (after ligation removal (IC + RL rats)) in immediate post-ligation time

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Transferase-mediated dUTP nick end-labeling (TUNEL) assay Apoptotic cells were detected by the TUNEL method using an in situ cell detection kit for the detection and quantification of apoptosis at a single-cell level