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Among these, the Apolipoprotein E (APOE) gene, particularly the 4 allele (APOE4), stands out as the most significant and consistent genetic risk factor

Gene expression profiles were detected using mouse lncRNA microarray analysis in the hippocampi of db/db mice

The column was then washed with 10 CV of 25 mM imidazole (Sigma-Aldrich) in DPBS (pH 7.2), followed by elution with 10 CV of 250 mM imidazole in DPBS (pH 7.3)

PE and GFP median fluorescence intensity values from GFP + BFP + cells were normalized by background subtraction of the respective values from reporter-negative cells