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Synthesized from glutamic acid, cysteine, and glycine, GSH serves a pivotal role in the pathway using niacinamide adenine dinucleotide to establish a reducing environment key for cellular function

Metabolite identity was established using a combination of an in-house metabolite library developed using pure purchased standards, the NIST library, and the Fiehn library

Future perspectives Lactate, a key participant in anaerobic metabolism, has increasingly become the focus of research, particularly regarding its bioavailability and production in various pathological and physiological states

Ioannis Mantas for his help with the illustration included in this Review

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