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In brief, cDNA was generated using M-MLV reverse transcriptase (Thermo Fisher) from RNA samples isolated from the cultures, and then incubated with Fast SYBR Green Master Mix (Applied Biosystems)

pgsh1 D:: HIS3MX6-pTEF1 / pgsh1 D:: HIS3MX6-pTEF1

The chronic disease code is sequenced first, followed by the D63 code