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Prav tako ni priporoljivo za osebe, obutljive na kofein (4 mg/100 ml)

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Bound proteins were eluted with His elution buffer (50 mM Tris-HCl pH 7.5, 50 mM NaCl, 250 mM imidazole, 2.5 mM ME)

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Serum IGF-I levels alone are usually insufficient for diagnosing AGHD, so provocative tests such as the insulin tolerance test, glucagon stimulation test, or the combined GHRH-arginine test (where available) are needed (28)