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In the MASLD mouse model of disordered lipid metabolism, the decline of CD4 + T cells in the liver can be related to the exacerbation of their apoptosis by high levels of fatty acids, whereas by inhibiting ROS, it is possible to restore the number of CD4 + T cells and to delay the tendency to transform into HCC (66)

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Zhang, X., Kapoor, D., Jeong, S., Fappi, A., Stitham, J., Shabrish, V., Sergin, I., Yousif, E., Yeh, Y., Park, A., Yurdagul, A., Rom, O., Epelman, S., Schilling, J

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We then systematically depleted vitamins in Plasmax medium for six days and utilized flow cytometry to assess proliferation changes using CountBright Absolute Counting beads or induction of myeloid differentiation using CD11b cell surface expression (Figure 1A, see Methods for media formulations)