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All animal procedures performed in this study were reviewed and approved by the Institutional Animal Care and Use Committee of the Zhejiang University School of Medicine and were conducted in accordance with the guidelines of the International Association for the Study of Pain

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Some cases also present with coma, visual impairment, progressive acoustic hearing loss and spastic paraplegia [101, 109]

A rotatory microtome was used to cut 5 m slices of each sample, which were subsequently placed on coated glass slides for H&E staining 49 or positive glass slides for immunohistochemical staining of P53 and caspase-3.The immune histochemistry protocol was followed according to the protocol of Petrosyan, et al

Vukojevi, J., Milavi, M., Perovi, D., Ili, S., ili, A

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