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Glutathione oxidation in response to intracellular H 2 O 2 : key but overlapping roles for dehydroascorbate reductases

Cell viability was measured using the CellTiterBlue reagent (Promega, United States) according to the manufacturers protocol with Fluoroskan Ascent FL Microplate Reader (Thermo Labsystems, Waltham, MA, United States)

doi: 10.3389/fneur.2018.00617, 143

[13] In the first step of the reaction, acyl-CoA synthetase catalyzes the transfer of adenosine monophosphate group (AMP) from an ATP molecule onto the fatty acid generating a fatty acyladenylate intermediate and a pyrophosphate group (PP i )

However, the vital importance of the ability of GSHee to overcome the negative effects of fentanyl on gas-exchange in the lungs cannot be over-emphasized

Lentivirus production and cell transduction The HBc sequence was cloned into the lentiviral vector pCDH-EF1-copGFP-T2A-Puro (Addgene, #72263), and the empty vector was used as a negative control