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There are several causes of vitamin B12 deficiency, including poor dietary intake, malabsorption, and pernicious anemia

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After reconstitution, the solution should remain refrigerated at 28C and protected from freeze-thaw cycles, which can cause peptide aggregation and measurable loss of bioactivity

Each 3ml vial is preserved with 0.9% Benzyl Alcohol to inhibit bacterial growth, ensuring solution stability for the duration of laboratory research cycles

In contrast, there is some evidence of an enhancement of the maximum absorption via intrinsic factor, by up to a factor of 2, when the vitamin B12 is accompanied by food, but this is not consistently observed and probably only applies when the food quantity and vitamin B12 dose are sufficient to slow the passage of the vitamin B12 through the small intestine enough (about three hours or more) to allow each intrinsic factor receptor to absorb two molecules of vitamin B12 from the oral dose rather than one