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Many NGP candidates are significant contributors to butyrate and propionate pools, thereby supplying energy substrates for colonocytes, enhancing epithelial barrier function, and exerting epigenetic and anti-inflammatory effects through HDAC inhibition and G-proteincoupled receptor signaling (96)

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LC-MS/MS analysis Peptides were separated using a loading column (100 m 2 cm) and a C18 separating capillary column (100 m 15 cm) packed in-house with Luna 3 m C18(2) bulk packing material (Phenomenex, USA)

MTS cell viability assay NIT-1 cells were seeded at 10,000 cells per well in 96-well plate and incubated for 24 h before treatment

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