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Kd values were obtained from binding curves using Prism 8.0 software (GraphPad Software)

For intracellular staining (ICS) 5,000,000 cells per well were plated in a 96-well round bottom plate (Costar 3799) and stimulated using the same ELISpot conditions as described above and incubated at 37C with 5% CO 2 for a total of 5.5h

At the same time, if verified peptide formulations were ultimately shown to enhance soft-tissue recovery without increasing infection risk, the clinical relevance for TJA would be substantial, underscoring the importance of rigorous clinical investigation rather than informal adoption

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The European Association of Urology/European Society for Paediatric Urology guidelines suggest that inguinal exploration may be avoided if blind ending vessels are observed during diagnostic laparoscopy