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The substrate is prepared by mixing gold nano-rods (AuNRs) (NR-40-650, NanoSeedz, Hong Kong), 3-amino-9-ethylcarbazole (AEC) (Sigma-Aldrich), HRP (Sigma-Aldrich), and agarose (Sigma-Aldrich) with final concentrations of 8.48 10 10 /ml, 0.125% (v/v), 1.25 g/ml, and 1.5% (w/v), respectively

Cold tolerance testing Mice were implanted subcutaneously in the dorsal region with IPTT-300 temperature transponders (BMDS)

doi: 10.16761/j.cnki.1000-4963.2025.02.015 [DOI] [PMC free article] [PubMed] [Google Scholar] 58

This, in turn, is associated with an increased risk of type 2 diabetes

For key races load with one to two shots daily for 3 to 7 days beforehand