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M.JacksonJ.WrenJ

Although Japan is on track for elimination of hepatitis C virus (HCV) by the World Health Organizations (WHO) 2030 goal, the epidemiology of HCV among PWID and MSM is very limited and poses a remaining challenge

Data 4 , 170045 (2017)

For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

The LGA parameters were set as follows: population size (ga_pop_size) = A, energy evaluations (ga_num_generation) = B, mutation rate = C, crossover rate = D, step size = E, and number of LGA runs = 10