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Placebo, 6.7 mg/L Zn and 0.6 mg/L of copper)

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Thirty or forty micrograms of proteins were loaded into each well and fractionated on 510% precast SDS-PAGE gels (Byeotime, Shanghai, China) and then transferred to a PVDF membrane (Merck Millipore, Darmstadt, Germany)

Second, as all analyses were exploratory, and the primary analysis for each endpoint was specified, multiple comparisons were performed only without P value correction

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