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Western blotting Neurons were lysed in sample buffer (1.5 M Tris, 3% SDS, 15% glycerol, 7.5% -mercaptoethanol, 0.0375% bromophenol blue, pH 6.8) and boiled for 4 min at 100 C

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[DOI] [PubMed] [Google Scholar] Zhou Y

Visibly reduces dark spots in 4 weeks*