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Following 6 min of Ca 2+ -free NT perfusion, the solution was exchanged for the Ca 2+ -free NT solution supplemented with 0.82 mg/mL collagenase (Type I), 0.5 mg/mL protease (type XIV 15% Ca 2+ ), and 1.67 mg/mL BSA prepared from factor V albumin (all purchased from Merck, Gillingham, United Kingdom) for 48 min until rod-shaped cardiomyocytes were visible in the perfusate

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The coach had vehemently defended himself against these charges in the media over the years, and on Tuesday, he said he would appeal the decision

Functions The bacteria in the gut affect the host in many ways

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