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MurF reaction: to 100 mM Tris buffer pH 7.9, 2.0 mM of one UDP-MurNAc-L-Ala-D-Glu-m-DAP derivative ( 1e 3e , Supplementary Information), 4.0 mM D-Ala-D-Ala (Sigma-Aldrich), 4.0 mM ATP and 2.0 mM MgCl 2 was added 1.0 g purified MurF enzyme per 100 l reaction sample

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