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The mean fluorescence intensity of CellROX was significantly higher in immune cells from TLS than those from the spleen or peripheral blood (Supplemental Figure 4)

Malik, S., Theis, M

Cells were harvested and centrifuged at 1200 rpm for 3 min, followed by incubation with 1 L annexin V-Alexa Fluor 488 (640906, BioLegend) in 100 L annexin V binding buffer 1 (10 mM HEPES (pH 7.4), 0.14 M sodium chloride (NaCl), 2.5 mM calcium chloride (CaCl 2 ) and incubated at room temperature in the dark for 15 min

TREATMENT Early initiation of treatment is critical immediately following recognition of DILI

L., Lusis, A

Materials and Methods Protein expression and purification GST-tagged GATE16 was expressed from the plasmid pGex-4T-2_GATE-16 (73518