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The G-site is formed by highly conserved amino acid residues in the N-terminal domain, while the H-site is formed by less conserved hydrophobic residues in the C-terminal domain

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There are two forms K1 (found in leafy greens) and K2 (found in fermented foods and animal products)

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The American Journal of the Medical Sciences

Inconsistent signals across plates are most commonly caused by temperature differences between the plate and reagent at the time of addition (ensure both are equilibrated to room temperature for 30 minutes before mixing), insufficient cell lysis (increase mixing time or speed), or signal read outside the stable window (read plates within 10 minutes to 1 hour after stabilization)