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Carbidopa is administered with levodopa to inhibit its early conversion to dopamine outside the central nervous system, thereby minimizing side effects like nausea and enhancing the quantity of levodopa penetrating the brain (47)

As a key antioxidant transcription factor, NRF2 activity decreases during heart failure, while miRNAs modulate NRF2 and its associated antioxidant proteins through EV, thereby regulating oxidative stress [166]

The deletion of Cth gene was confirmed by qPCR in ECs isolated from lungs as previously described [8]

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The crude product was purified using a flash chromatography silica gel column

Encapsulation efficiencies were 51.71 0.54% for PX and 65.15 2.30% for Au nanospheres