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Potential side effects may include: Nausea Stomach discomfort Mild diarrhea These effects are usually temporary and improve with dosage adjustments

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The next day, cells were collected and washed with PBS and solubilized in a buffer containing Brij 97 (1% Brij 97, 25 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA and phosphatase and protease inhibitors) for 30 min with regular agitation at 4 C

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Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol