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Organoids were cultured in growth media consisting of advanced DMEM/F12 medium (Invitrogen) containing B-27 (Invitrogen, USA), 1.25 mM N-acetylcysteine (Sigma), 10 mM nicotinamide (Sigma), 10 mM HEPES (Gibco), 1% GlutaMAX (Gibco), 50 ng/mL EGF (PeproTech, USA), 100 ng/mL FGF-10 (Peprotech), 50 ng/mL recombinant human R-spondin 1 (Qkine), 100 ng/mL WNT surrogate-Fc fusion protein (U-protein express BV), 1% noggin-Fc fusion protein-conditioned medium (U-protein express BV), 500 nM A83-01 (Tocris), and 1% primocin (InvivoGen) were added

doi: 10.1016/j.foodchem.2021.131908 9

Meanwhile, in APAP-treated primary hepatocytes obtained from Ddit4 Hep mice, DDIT4 deficiency diminished PGC-1 expression induced by AMSCs (Fig

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E., Skarlupka, J