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NFS1 and ISCU form a heterodimeric complex (Figure 2B, reaction 2) in which the persulfide generated by NFS1 (Figure 2B, reaction 3) is transferred to ISCU when iron is present in the assembly site of ISCU (Figure 2B, reaction 4)

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2.8 UHPLC-QTOF-MS analysis of bael gum Bael gum was further analyzed using the UHPLC-QTOF-MS technique as per the procedure described by Haron et al

To test this, we first assessed cytoplasmic free mtDNA levels under hypoxia using immunofluorescence, and M9a+sh EPAS1 cells exhibited significantly reduced cytoplasmic free mtDNA compared to both M9a+shCTR and M7/8+shCTR cells (Fig