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The mutant protein was purified by using BeyoGold TM His tag purification resin (denaturant resistant) (P2233, Beyotime Biotechnology, Shanghai, China) according to the manufacturer's protocol

Regulating TAM iron metabolism within the TIME, specifically by promoting iron uptake and storage in M2-type macrophages while inhibiting iron release, allows iron accumulation in TAMs to enhance ROS production, increase p300/CBP acetyltransferase activity, and promote p53 acetylation

Finally, cancer-associated fibroblasts (CAFs) can be activated by ROS and inflammation in the tumor microenvironment 173 and are also consistently implicated in tumor progression by supporting tumor growth and invasion through secretion of cytokines and extracellular matrix remodeling components 174

Both FRDA fibroblast cell lines showed reduced expression levels of the mitochondrial subunits, NDUFS1 and SDHB compared to control, which could be explained by the Fe-S cluster deficit

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