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The cells were washed twice and starved for 1 h in Hanks Balanced Salt Solution (HBSS) (ThermoFisher)

Regarding material design, incubation studies in phosphate-buffered saline (PBS) demonstrated that HA coating substantially enhanced MPH NPs stability ( Figure 6B ), while acidic conditions and elevated HAase concentrations accelerated PMB release ( Figure 6C ), confirming superior targeting performance

Since growth hormone release is regulated by the hypothalamus and pituitary gland, any influence on these neural systems might indirectly affect testosterone production

This approach can be further developed into real-time decision-support systems in clinical settings, where physicians dynamically adjust treatments based on patient responses

It can be as little as 20% of the volume of a regular single session, or as much as 40%

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