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Patenaude, J., DElia, M., Cote-Maurais, G

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Measurement of the concentration of macro and micro elements The wet digestion method was used to measure the concentration of macro and microelements 27 for which 500 mg of dry and ground leaf samples were digested in a mixture of sulfuric acid (H 2 SO 4 ) and nitric acid (HNO 3 ) at a ratio of 1:5 (v/v) at 60 C for 24 h

Blots were then incubated with secondary antibody for one hour at room temperature

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