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for Biotechnology Spectrum Diagnostics, Al-Obour city, Egypt, 226,002, 294,002 for calcium and phosphorus, respectively) were estimated according to the o-cresolphthalein complex-one and UV-phosphomolybdate methods, respectively

The primers were designed in PrimerBank 67

Addo KA, Bulka C, Dhingra R, et al

Rescue of tolerant CD8+ T cells during cancer immunotherapy with IL2:antibody complexes

After incubation, plates were centrifuged at room temperature for 10 min at 350 g and a tetrazolium dye MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (Merck, Cat No