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Some were randomized controlled trials (RCT, double-blinded or no-blinded), some were one-group or two-group intervention studies with Se, without a control group, or controlled with another treatment

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Vitamin C mechanisms involve serving as enzymatic cofactor for collagen hydroxylation and providing direct antioxidant protection, creating effects requiring ongoing application for maintaining antioxidant benefits but potentially supporting durable collagen improvements if used sufficiently long

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