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Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) were tested on 3 different plates, 8 replicates in each plate

Reverse transcription-polymerase chain reaction (RT-PCR) was conducted using a RevertAid First Strand cDNA Synthesis Kit (ThermoFisher Scientific, Waltham, MA, USA) and subsequent real-time quantitative PCR was performed using a TB Green Premix Ex Taq TM (Tli RNaseH plus) Kit (TaKaRa Biology (Dalian), Liaoning, China)

Both were reasonable choices fifteen years ago

Similar to the majority of chronic diseases (for instance, diabetes), there is no cure for opioid use disorder, or any other addiction

The IECs were collected by centrifugation at 250 g for 10 min and stored at 80C for further analysis

PMID: 34187083