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To calculate the percentage inhibition, the differential signal activated by calcium in the presence of compounds was expressed in relationship to that observed in DMSO wells (100% activation) and to the GFPSTX7:FlagMunc13-4-C2A*B* mutant control (0% activation)

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PDL coating involved adding 100 g ml 1 PDL in sterile H 2 O for 30 min, followed by two washes with H 2 O and air-drying

doi: 10.1186/s13073-016-0303-2 28 BretonJ.TennouneN.LucasN.FrancoisM.LegrandR.JacquemotJ.et al

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