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GSH reacts with DTNB to form compounds with characteristic absorption peaks at 412 nm

Sections were then incubated with anti-NF-B p65 monoclonal antibody (Catalog No: (F-6): sc-8008, Santa Cruz Biotechnology, USA, 1:100 dilution), anti-PPAR- monoclonal antibody (Catalog No: (E-8): sc-7273, Santa Cruz Biotechnology, USA, 1:200 dilution), anti-caspase-3 polyclonal antibody (Catalog No: PA5-77887, Invitrogen, USA, 1:100 dilution), anti-pErk-1 polyclonal rabbit antibody (Catalog No: PA5-40294, Invitrogen, USA, 1:20 dilution), and anti-PECAM-1 monoclonal antibody (Catalog No: (H-3): sc-376,764, Santa Cruz Biotechnology, USA, 1:200 dilution)

Quantification of GSH using LCMS Cells were incubated with 100 M of N -methyl maleimide (NMM) in PBS for 15 min at room temperature to derivatize all the small-molecule thiols

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Quercetin Attenuates Trauma-Induced Heterotopic Ossification by Tuning Immune Cell Infiltration and Related Inflammatory Insult

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