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Briefly, 10 ml of 0.1 M potassium phosphate buffer (pH 6.8) was used to grind and homogenize leaf tissue subjected to salt stress, as well as control plants

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Thereafter, the cells were subjected to various treatments: PBS, PBS + Laser, 28.8 g/mL PDA, 40 g/mL PDA@Pt, and 40 g/mL PDA@Pt + Laser (1 W/cm 2 , 5 min)

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Depression is associated with increased inflammation and given that HPA axis is strictly implicated in inflammation, it is hypothesized that alteration in HPA axis is associated with increase in pro-inflammatory cytokines causing depression, at least with a subgroup of individuals with depression (249)