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GraphPad Software, San Diego, USA) was used to create the figures

Shaking introduces violent mechanical forces that can break the bonds holding the semaglutide molecule in its active conformation

If you freeze, use small sterile vials or tubes

Measurement of cellular iron Cell iron content assay kit (Solarbio, Beijing, China) was used to measure cellular iron contents based on assay contains hydroxylamine hydrochloride, which reduces Fe 3+ to Fe 2+ in cells, and under weak acid conditions, Fe 2+ and phenoline form a reddish-orange mixture, and the iron content can be calculated by measuring the OD value at 510 nm

Acknowledgments UF thank the Indian Council of Medical Research-Department of Health Research (ICMR-DHR) for the award of Young Scientist

CagriSema versus tirzepatide in people with type 2 diabetes