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mobile phase is the triethylamine buffer solution (8ml of phosphate, 15ml of triethylene amine, 1500ml of water, pH adjusted to 5.4) - tetrahydrofuran(THF) mixture, the time gradient is listed in the following Table 2: Table 2 HPLC gradient time table Time(min) Triethylamine buffer solution (%) THF (%) 0 75 25 10 75 25 11 30 70 18 30 70 19 75 25 25 75 25 Step 1 Preparation of L-3-chloro-2-hydroxypropyl trimethylammonium Firstly, the content of L-isomer of S-epichlorohydrin was detected according to the method of example 1 above

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