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Similarly, resistance to oxidative attack increased after overexpression of GPX5 in CHO-K1 cells, and GPX5 overexpressing cells reduced lipid peroxidation levels and downstream DNA damage marker 8-OXODG levels compared to control cells (Taylor et al., 2013)

This method has been used to extract polyphenols from green tea, laurel leaves, cannabis, and Phyllanthus emblica , with extracts showing anti-inflammatory and antioxidant activity (70, 71)

Others reported studies using AD mouse models fed a diet including deuterated 11,11-D 2 -linoleic acid ethyl ester (D 2 -LA) and 11,11,14,14-D 4 -alpha-linolenic acid ethyl ester (D 4 -ALA) in their diets for a sufficient period such that the phospholipids in brain became rich in deuterated fatty acids with allylic deuterium atoms in the indicated unsaturated fatty acids of phospholipids (127, 128)

Complementary mechanisms

Long-term ethanol feeding indirectly deactivated GPx4 through severe GSH exhaustion GPx4 is the only phospholipid hydroperoxide scavenger that protects cells from membrane disruption

direct evidence for IV vitamin C reducing OA-specific inflammatory markers in high-quality human trials is limited