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Sasaki et al., 2017)

Carrico C, Meyer JG, He W, Gibson BW, Verdin E

The cells were maintained at 37C in an atmosphere of 5% CO2 in RPMI-1640 (Thermo Fisher Scientific, Inc., Waltham, MA, USA) with 10% fetal bovine serum (FBS

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Briefly, 5.0 10 5 cells were transfected with 1.5 g of DNA plasmid and 3 L of Lipofectamine 2000 in a 12-well plate setting according to the manufacturers instructions

The proposed mechanism is that in the absence of efficient GST-mediated conjugation, SFN is cleared more slowly, allowing it to circulate in its active form for a longer duration and potentially exert stronger biological effects (57)