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ER Ca 2+ release, sustained store-operated Ca 2+ entry, and persistent cytosolic Ca 2+ elevation can continue to damage PACs even when mitochondrial Ca 2+ entry through MCU is reduced, because calcium-dependent digestive enzyme activation, PM injury, and failure of ATP-dependent ion pumps remain operative

Then, the resulting data matrixes were imported into SIMCA 14.0 software (Umetrics, Sweden) for multivariate data analysis, including principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA)

The researchers conclude: Our findings, together with those of previous studies (7, 8, 19, 20), suggest that the current RDA of 2.4 mcg vitamin B-12/d may be inadequate for optimal biomarker status

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We believe that these are through local iron overload (Toyokuni, 2013b)