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Following internalization, the receptor is retained and recycled to the plasma membrane as a resensitized receptor or, upon recognition of an interaction with the GPCR-associated sorting protein (GASP), the receptor will be sent to the lysosome for degradation (33,43)

The dosage may range from 1,000 to 4,000 IU (two to three times per week) for several weeks or months

H., Yuan, L

doi: 10.1007/s00726-013-1481-5

the additional time and the high cost associated with proteases needed for tag removal have greatly hampered the usefulness of affinity tags in large-scale industrial processes, as no simple and low-cost method exists to date for affinity tag removal

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