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MALDI-MS CSF N-glycome profiling enabled detection of peculiar changes in subject affected by neurodegenerative diseases, helpful in monitoring diseases development and progression, thus representing a source of potential biomarkers and therapeutic targets

Starting from day 6 we included 10 M cyclopamine to promote dorsalization

To gauge insight into secretion dynamics of the individual proteins, average NSAF values (at least two of the three runs) of individual proteins were graphed using PRISM 8 (GraphPad Software, San Diego, CA)

Therefore, specific primer design requirements must be met for DNA sequences

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Overall, further research is necessary to establish the stability of the models in terms of consistency and reproducibility