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Dissociative anesthetics, including ketamine and nitrous oxide, impair glutamatergic transmission and mitochondrial function, thereby exacerbating excitotoxicity and neuronal apoptosis

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For the proliferation assay, 210 5 cells per well in 48 wells plates (Corning) were cultured with DMEM media, supplemented with 10% FBS as well as sodium butyrate at the 5M and 10M concentrations