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For immunohistochemistry (IHC), antigen retrieval was performed on rehydrated sections and sodium citrate buffer (10 mM, pH 6.0) in a pressure cooker at high pressure for 5 minutes was used for F4/80, while Tris-EDTA buffer (10 mM, pH 9.0) at high pressure for 10 minutes was used for PCNA and Ly6g