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Protein lysates (30 g) were individually heated at 95C for 10 min in 2X Laemmli sample buffer (1:1 ratio) and resolved by electrophoresis in 10% sodium dodecyl sulfate (SDS)polyacrylamide gel using Mini-PROTEAN electrophoresis tank (Bio-Rad, United States) at 100 V for 90 min in 1X Running Buffer (Tris-glycine SDS, pH 8.3, Bio-Rad, United States)

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When treatment stops, the biological systems that regulated hunger before therapy resume their original patterns

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A brief overview of the current findings