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In spite of the perceived hypotheses regarding the benefits of antioxidant supplementation on pregnancy outcomes, clinical trials investigating the use of antioxidants to treat reproductive disorders have reported largely conflicting results

[PMC free article: PMC3306697] [PubMed: 22371576] 100

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Free thiol groups were then blocked using a buffer that contained 25 mM 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid, pH 7.4, 0.1 mM EDTA, pH 8.0, 0.01 mM neocuproine (Sigma-Aldrich, N1501), 40 mM N -ethylmaleimide (Sigma-Aldrich, E1271), and 1% Triton (Sigma-Aldrich) for 30 min

Bott et al, 2015)

PMID: 26406389 Potga, A