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Determination of intracellular reactive oxygen species (ROS) levels Cells were grown in dark, clear bottom 96-well microplates and treated for 96 h with the desired conditions

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Maintaining an energy deficient diet during training often leads to a number of physical (i.e., loss of fat-free mass, illness, reduced sleep quality, incomplete recovery, hormonal fluctuations, increased resting heart rate, etc.) and psychological (i.e., apathy towards training, heightened stress) adverse outcomes [23, 27]