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Infarct size was assessed after 30 min of ischaemia followed by 24 h of reperfusion using Evans blue staining (1%) for delineation of the ischaemic area at risk (AAR) from the non-ischaemic zone (remote area) and 1% 2,3,5-triphenyl tetrazolium chloride (TTC) staining for demarcation of the viable and non-viable myocardium within the AAR, as described 19

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The different sizes, gross shapes, and histological structures of layers that separate maternal and fetal circulations result in various types of placentas

doi:10.1046/j.0022-202x.2001.01469.x