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Brit Med Bull 49: 481493

Notably, SLC38A9 is necessary for not only leucine but also other essential amino acids (e.g., glutamine, tyrosine, and phenylalanine) that are generated via lysosomal proteolysis and efflux from lysosomes in an arginine-stimulated fashion to activate mTORC1 (Rebsamen et al., 2015

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In addition, high-density intensive culture leads to a significant decrease in dissolved oxygen concentration in water, creating a hypoxic environment