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harvested cells were first re-suspended in chilled lysis buffer (20 mM Tris [pH 7.9], 0.5 M NaCl, 5 mM imidazole, 10% glycerol, 1 mM DTT, 1 mM PMSF) and the lysis mixture was sonicated on ice

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These approaches can help bridge the gap between theoretical aspirations and practical implementation, fostering a more inclusive and resilient urban future

The tight coupling between the subunits of new proteins in the ER lumen and the ER folding ability is necessary for effective protein folding in ER [88]